Abstract: This paper introduced the NNSA, IAEA and NRC requirements for first of a kind test of nuclear power plants, and determined the design philosophy of the first of a kind commission test of HPR1000 combined with the operation experience feedback of nuclear power plants and engineering practice of similar nuclear power plants.  The first of a kind test items of HPR1000 is researched and determined, based on the analysis of the new design concept and new design feature used in HPR1000. The test condition, test content and test acceptance criteria of the first of a kind test items were analyzed one by one, in order to guide the First of a Kind Test of HPR1000.