Abstract: To verify the structural integrity and operability under the seismic condition required by the third generation of nuclear power plants, the seismic test of ACP1000 control rod drive line（CRDL） was conducted on the multi-exciters test unit. One horizontal direction and one vertical direction of the CRDL were excited with multi-frequency earthquake time history. Drop-time of CRDL under earthquake was obtained. The acceleration and strain response values of CRDL under OBE and SSE level were measured. Operation functions under OBE level and safety function under SSE level of CRDL were validated. The seismic test shows that ACP1000 CRDL can keep the structural integrity and operability under the seismic condition.