Abstract: An experimental study on hydraulic characteristics of Tube Support Plate（TSP） with different holes shaped like broached-trefoil and broached-quatrefoil was carried out under the conditions of single-phase and two-phase flow. The results show that the single-phase local resistance coefficient of TSP decreases with the increasing of Reynolds number, and becomes constant afterwards. The bigger the radius of the chamfer of the hole or the ratio of the flow area in the TSP and the tube bundle, the smaller the single-phase local resistance coefficient of TSP. The local pressure drop is slightly larger than the calculation values by the correlations of Chisholm and Lin Zhonghu, and coincides well with the results by Computational Fluid Dynamics（CFD）.