Abstract: The two way coupling numerical model for a typical pipe conveying fluid is established based on two way coupling method with CFD and FEM, and the dynamic characteristics of flow-induced vibration are obtained. The effects of flow velocity, stiffness and location of pipe supports on its flow-induced vibration characteristics are studied. It can conclude that the low frequency vibration can be excited by hydrodynamic loading, and the low frequency line spectrum is significant. The flow-induced vibration of pipe is closely related to the flow velocity inside, the stiffness and location of pipe supports. There is a positive correlation between the flow velocity of fluid inside and the total flow-induced vibration level of the pipe. The effect of stiffness and location of pipe supports on its spectrum distribution characteristics for flow-induced vibration is significant, and the resonance frequency of equipment and valve can be avoided by optimizing the parameters of pipe supports.