Abstract: In order to obtain the response spectrum for the specific nuclear power plant (NPP), considering the specific site condition and ground motion parameters, the stochastic simulation and probabilistic hazard analysis is combined to obtain the UHS which the probability of exceedance is 10^-4. In order to verify the seismic performance and applicability of the spectrum in the actual structure, 1:20 NPP model is designed for shaking table test, comparing the structure response of two natural waves and two artificial waves which are generated by the safety evaluation spectrum and RG1.60 spectrum. The result shows that different wave has a different seismic isolation effect. The artificial wave generated by the UHS has a great effect on the acceleration amplification and the displacement of the NPP. The floor response spectrum amplitude which caused by UHS is higher than other waves. It should be considered in the structure and equipment seismic design.