Neutron activation analysis and mass spectrometry are used as 235 U isotope abundance of fuel assembly. Neutron activation analysis and mass spectrometry can’t be used as an effective measurement means of the 235 U Isotope Abundance of Fuel Assembly. Meanwhile they aren’t also used as a verification mean of 235 U isotopic abundance the fuel assembly management and transportation because of their shortcomings of large measurement equipment and sampling. So based on a compact neutron generator, a rapid and portable measurement method for 235 U Isotope Abundance of Fuel Assembly is significant. According to the linear correlation relating the ratio of average yield for the specific fissile nuclides to 235 U Isotope Abundance, the samples whose 235 U Isotope Abundance is 10%~90% are successfully measured. It provides the experimental basis for the verification of 235 U Isotope Abundance of the fuel assembly for the research reactor.