In the 5th and 11th fuel cycle of Qinshan NPP,the core was loaded with an over decayed neutron source,and without irradiation activated neutron source respectively,which caused a blind area of ex-core nuclear detecting instrumentation.This paper introduces the Chinese regulatory requirements and the corresponding measurements which Qinshan NPP took to apply the reactor start-up without neutron source,and shows that the safety of reactor start-up without neutron source can be ensured.