A theoretical model is built to calculate the location of fluid stratified interface in density lock,the calculated values and experimental observations are compared and the difference between them are analyzed.The effect of disturbance velocity and temperature difference on the interface location is discussed,respectively.The result shows that the larger disturbance velocity responds to the lower interface location,and the higher temperature difference responds to the higher interface location.In condition of certain temperature and diameter,the experiential relationships among the interface location,disturbance velocity and Richardson number are fitted,respectively.