Experimental studies of heat transfer in the start up of density lock are carried out, respecitvelywithout disturbance and with different disturbances. The research indicates that, stratification can be dividedinto three types, i.e., strong stratification, weak stratification and no stratification. Both the former two casescan restrain heat transfer, moreover, the heat transfer in strong stratification is less than that in week stratification and the heat would directly go through the density lock continuously in the third case so that the density lock is invalid.