The anti-disturbance mechanism of the density lock is analyzed by cold and hot state experi-ments with theoretical analysis.The result shows that in cold state,the pressure difference which caused by the fluid flow will produce the large circulation of fluid movement in channels of density lock.And in hot state,the formation of fluid stratified interface in the density lock will stop the large circulation of fluid movement.The further study shows that as a result of pressure difference,the geopotential difference is pro-duced by the slope of fluid stratified interface,and when the pressure difference is equal to the geopotential difference,the large circulation of fluid movement is stopped.